Scripts

Scripts#

Fishtank provides a number command line scripts to help with common tasks. These scripts are installed when you install fishtank. You can see the list of available scripts by running fishtank --help.

scripts.align_experiments(ref, moving, ...)

Align experiments using optical flow.

scripts.aggregate_polygons(input[, output, ...])

Aggregate polygons from multiple FOVs.

scripts.assign_proseg(transcripts, ...[, ...])

Assign additional transcripts to cells using ProSeg.

scripts.assign_spots(input[, polygons, ...])

Assign spots to the nearest polygon.

scripts.cellpose(input, fov, channels[, ...])

Segment cells using Cellpose.

scripts.decode_spots(input[, strategy, ...])

Decode spots using specified strategy.

scripts.detect_spots(input, fov, ref_series, ...)

Detect spots in an image and quantify their intensity.

scripts.fovs(input[, file_pattern])

List fields of view in an input directory.